Figure 1b.pptx

Figure 1: Expression of US and MS RNA and HIV DNA in total and resting CD4+ T cells isolated from PLWH on suppressive ART. (a) US and MS HIV RNA and HIV DNA were quantified in freshly isolated total (solid symbols) and resting (open symbols) CD4+ T cells. p values were determined by paired t test on logarithmically transformed data. All data are shown as the mean +/- SEM. Each symbol represents a single donor. N = 6. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (b) Schematic alignment of US and MS RNA primer sequences in reference to the HIV genome and HXB2 sequence. HIV DNA quantification was a single PCR amplification and used SL19 and SL20 (c) The percent of each primer or primer pair that is a complete match, contains a base pair mismatch(es) or is deleted in 993 representative defective proviral sequences obtained from the proviral sequence database (34).