Figure 2: Changes in US, MS and SN RNA following stimulation of total and resting CD4+ T cells with LRAs after 3 days. Total and resting CD4+ T cells from PLWH on ART were stimulated for 3 days with different LRAs and (a) US RNA and (b) MS RNA were quantified. The fold change from DMSO at day 3 is shown. (c) The ratio of MS/US RNA was calculated using the raw RNA copy numbers. (d) SN RNA was quantified 3 days following stimulation with different LRAs. All data are shown as the mean +/- SEM. Each symbol represents a single donor. N = 6. p values were calculated using a one sample t test on logarithmically transformed data comparing each LRA to the DMSO control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.