Nanopore sequencing

Figure 3: Frequency per million of known CALCR transcripts expressed in HGG cell lines treated with 1 µM staurosporine across the time-period of 30 hr. The expected length of the barcoded cDNA amplicon samples is 3.4 kb, reads with quality score below 10 were excluded and only transcripts with a minimum occurrence of 500 times were included. Panels A-C show the frequency per million of the two transcripts (transcript 2 CT_a Receptor and transcript 2 CT_b Receptor) expressed over the 30 hr time-period. The error bars represent +/- SD from three individual experiments (n=3). BL = baseline.